PeptideCutter Report

seq_1

Length 14 aa · Enzymes 38

Part 1Input Sequence

           10
 1 KIDWCHEIQPSSVR

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	14
Asp-N endopeptidase	1	2
Asp-N endopeptidase + N-terminal Glu	2	2, 6
BNPS-Skatole	1	4
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	4
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	2	4, 6
Clostripain (Clostridiopeptidase B)	1	14
Formic acid	1	3
Glutamyl endopeptidase	1	7
Iodosobenzoic acid	1	4
LysC	1	1
Neutrophil elastase	1	13
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	4
Pepsin (pH>2)	1	4
Proteinase K	5	2, 4, 7, 8, 13
Staphylococcal peptidase I	1	7
Thermolysin	2	1, 12
Trypsin	1	1

The selected enzymes do not cut: Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, CNBr, Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), LysN, Pepsin (pH1.3), Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                          ArgC_Clost|
                                        Elast_ProtK||
                                             Therm|||
                                         ProtK|   |||
                             GluC_ProtK_Staph||   |||
                          AspN_AspGluN_Ch_lo|||   |||
     BNPS_Ch_hi_Ch_lo_Iodo_NTCB_Pn2p_ProtK| |||   |||
                                    HCOOH|| |||   |||
                 AspN_AspN_AspGluN_ProtK||| |||   |||
                       LysC_Therm_Tryps|||| |||   |||
                                       KIDWCHEIQPSSVR
                                   1   ---------+----   14

seq_2

Length 18 aa · Enzymes 38

Part 1Input Sequence

           10
 1 LWEAFMSPHHFEKCKWYE

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase + N-terminal Glu	3	2, 11, 17
BNPS-Skatole	2	2, 16
Chymotrypsin-high specificity (C-term to [FYW], not before P)	5	2, 5, 11, 16, 17
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	9	1, 2, 5, 6, 9, 10, 11, 16, 17
CNBr	1	6
Glutamyl endopeptidase	3	3, 12, 18
Iodosobenzoic acid	2	2, 16
LysC	2	13, 15
LysN	2	12, 14
Neutrophil elastase	1	4
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	13
Pepsin (pH1.3)	3	4, 5, 10
Pepsin (pH>2)	4	4, 5, 10, 16
Proteinase K	10	1, 2, 3, 4, 5, 11, 12, 16, 17, 18
Staphylococcal peptidase I	3	3, 12, 18
Thermolysin	3	4, 5, 10
Trypsin	2	13, 15

The selected enzymes do not cut: Arg-C proteinase, Asp-N endopeptidase, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), Enterokinase, Factor Xa, Formic acid, GranzymeB, Hydroxylamine (NH2OH), Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                           GluC_ProtK_Staph|
                            AspN_AspGluN_Ch_hi_Ch_lo_ProtK||
                         BNPS_Ch_hi_Ch_lo_Iodo_Pn2p_ProtK|||
                                              LysC_Tryps||||
                                                   LysN|||||
                                       LysC_NTCB_Tryps||||||
                                GluC_LysN_ProtK_Staph|||||||
                      AspN_AspGluN_Ch_hi_Ch_lo_ProtK||||||||
                             Ch_lo_Pn1.3_Pn2p_Therm|||||||||
                                             Ch_lo||||||||||
                                     Ch_lo_CNBr|  ||||||||||
            Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK_Therm||  ||||||||||
                 Elast_Pn1.3_Pn2p_ProtK_Therm|||  ||||||||||
                            GluC_ProtK_Staph||||  ||||||||||
   AspN_AspGluN_BNPS_Ch_hi_Ch_lo_Iodo_ProtK|||||  ||||||||||
                               Ch_lo_ProtK||||||  ||||||||||
                                          LWEAFMSPHHFEKCKWYE
                                      1   ---------+--------   18

seq_3

Length 12 aa · Enzymes 38

Part 1Input Sequence

           10
 1 SAFDKCTKAYNK

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase	1	3
Asp-N endopeptidase + N-terminal Glu	1	3
Chymotrypsin-high specificity (C-term to [FYW], not before P)	2	3, 10
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	2	3, 10
Formic acid	1	4
LysC	3	5, 8, 12
LysN	3	4, 7, 11
Neutrophil elastase	2	2, 9
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	5
Pepsin (pH1.3)	1	3
Pepsin (pH>2)	2	3, 9
Proteinase K	5	2, 3, 7, 9, 10
Thermolysin	3	1, 2, 8
Trypsin	2	5, 8

The selected enzymes do not cut: Arg-C proteinase, BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), CNBr, Enterokinase, Factor Xa, Glutamyl endopeptidase, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, Proline-endopeptidase[*], Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                                       LysC|
                                                      LysN||
                                        Ch_hi_Ch_lo_ProtK|||
                                        Elast_Pn2p_ProtK||||
                                       LysC_Therm_Tryps|||||
                                            LysN_ProtK||||||
                                     LysC_NTCB_Tryps| ||||||
                                         HCOOH_LysN|| ||||||
    AspN_AspN_AspGluN_Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK||| ||||||
                                Elast_ProtK_Therm|||| ||||||
                                           Therm||||| ||||||
                                                SAFDKCTKAYNK
                                            1   ---------+--   12

seq_4

Length 14 aa · Enzymes 38

Part 1Input Sequence

           10
 1 SHQTTHPWMWKNAN

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
BNPS-Skatole	2	8, 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	10
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	3	2, 9, 10
CNBr	1	9
Iodosobenzoic acid	2	8, 10
LysC	1	11
LysN	1	10
Neutrophil elastase	1	13
Pepsin (pH>2)	3	7, 9, 10
Proline-endopeptidase[*]	1	7
Proteinase K	5	4, 5, 8, 10, 13
Thermolysin	2	8, 12
Trypsin	1	11

The selected enzymes do not cut: Arg-C proteinase, Asp-N endopeptidase, Asp-N endopeptidase + N-terminal Glu, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), Enterokinase, Factor Xa, Formic acid, Glutamyl endopeptidase, GranzymeB, Hydroxylamine (NH2OH), NTCB (2-nitro-5-thiocyanobenzoic acid), Pepsin (pH1.3), Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                        Elast_ProtK|
                                             Therm||
                                       LysC_Tryps|||
           BNPS_Ch_hi_Ch_lo_Iodo_LysN_Pn2p_ProtK||||
                                Ch_lo_CNBr_Pn2p|||||
                         BNPS_Iodo_ProtK_Therm||||||
                                    Pn2p_Prol|||||||
                                      ProtK| |||||||
                                     ProtK|| |||||||
                                   Ch_lo| || |||||||
                                       SHQTTHPWMWKNAN
                                   1   ---------+----   14

seq_5

Length 12 aa · Enzymes 38

Part 1Input Sequence

           10
 1 PFFWIILKHNTP

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
BNPS-Skatole	1	4
Chymotrypsin-high specificity (C-term to [FYW], not before P)	3	2, 3, 4
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	5	2, 3, 4, 7, 9
Iodosobenzoic acid	1	4
LysC	1	8
LysN	1	7
Pepsin (pH1.3)	3	3, 6, 7
Pepsin (pH>2)	4	3, 4, 6, 7
Proteinase K	7	2, 3, 4, 5, 6, 7, 11
Thermolysin	5	1, 2, 4, 5, 6
Trypsin	1	8

The selected enzymes do not cut: Arg-C proteinase, Asp-N endopeptidase, Asp-N endopeptidase + N-terminal Glu, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), CNBr, Enterokinase, Factor Xa, Formic acid, Glutamyl endopeptidase, GranzymeB, Hydroxylamine (NH2OH), Neutrophil elastase, NTCB (2-nitro-5-thiocyanobenzoic acid), Proline-endopeptidase[*], Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                             ProtK|
                                           Ch_lo| |
                                     LysC_Tryps|| |
                   Ch_lo_LysN_Pn1.3_Pn2p_ProtK||| |
                       Pn1.3_Pn2p_ProtK_Therm|||| |
                                 ProtK_Therm||||| |
     BNPS_Ch_hi_Ch_lo_Iodo_Pn2p_ProtK_Therm|||||| |
              Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK||||||| |
                  Ch_hi_Ch_lo_ProtK_Therm|||||||| |
                                   Therm||||||||| |
                                        PFFWIILKHNTP
                                    1   ---------+--   12

seq_6

Length 13 aa · Enzymes 38

Part 1Input Sequence

           10
 1 MQARHTDQWDTKS

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	4
Asp-N endopeptidase	2	6, 9
Asp-N endopeptidase + N-terminal Glu	2	6, 9
BNPS-Skatole	1	9
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	9
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	3	1, 5, 9
Clostripain (Clostridiopeptidase B)	1	4
CNBr	1	1
Formic acid	2	7, 10
Iodosobenzoic acid	1	9
LysC	1	12
LysN	1	11
Neutrophil elastase	1	3
Pepsin (pH>2)	2	8, 9
Proteinase K	4	3, 6, 9, 11
Thermolysin	1	2
Trypsin	2	4, 12

The selected enzymes do not cut: Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Enterokinase, Factor Xa, Glutamyl endopeptidase, GranzymeB, Hydroxylamine (NH2OH), NTCB (2-nitro-5-thiocyanobenzoic acid), Pepsin (pH1.3), Proline-endopeptidase[*], Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                                     LysC_Tryps|
                                                    LysN_ProtK||
                                                        HCOOH|||
          AspN_AspN_AspGluN_BNPS_Ch_hi_Ch_lo_Iodo_Pn2p_ProtK||||
                                                       Pn2p|||||
                                                     HCOOH||||||
                                  AspN_AspN_AspGluN_ProtK|||||||
                                                   Ch_lo||||||||
                                       ArgC_Clost_Tryps|||||||||
                                           Elast_ProtK||||||||||
                                                Therm|||||||||||
                                          Ch_lo_CNBr||||||||||||
                                                    MQARHTDQWDTKS
                                                1   ---------+---   13

seq_7

Length 13 aa · Enzymes 38

Part 1Input Sequence

           10
 1 GQELGYLELHLID

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase	1	12
Asp-N endopeptidase + N-terminal Glu	3	2, 7, 12
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	6
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	6	4, 6, 7, 9, 10, 11
Formic acid	1	13
Glutamyl endopeptidase	2	3, 8
Pepsin (pH1.3)	8	3, 4, 6, 7, 8, 9, 10, 11
Pepsin (pH>2)	9	3, 4, 5, 6, 7, 8, 9, 10, 11
Proteinase K	8	3, 4, 6, 7, 8, 9, 11, 12
Staphylococcal peptidase I	2	3, 8
Thermolysin	3	6, 10, 11

The selected enzymes do not cut: Arg-C proteinase, BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), CNBr, Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, LysC, LysN, Neutrophil elastase, NTCB (2-nitro-5-thiocyanobenzoic acid), Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease, Trypsin

Part 4Cleavage Mapping

                                            HCOOH|
                         AspN_AspN_AspGluN_ProtK||
                   Ch_lo_Pn1.3_Pn2p_ProtK_Therm|||
                        Ch_lo_Pn1.3_Pn2p_Therm||||
                       Ch_lo_Pn1.3_Pn2p_ProtK|||||
                 GluC_Pn1.3_Pn2p_ProtK_Staph||||||
        AspN_AspGluN_Ch_lo_Pn1.3_Pn2p_ProtK|||||||
        Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK_Therm||||||||
                                     Pn2p|||||||||
                  Ch_lo_Pn1.3_Pn2p_ProtK||||||||||
            GluC_Pn1.3_Pn2p_ProtK_Staph|||||||||||
                          AspN_AspGluN||||||||||||
                                     GQELGYLELHLID
                                 1   ---------+---   13

seq_8

Length 15 aa · Enzymes 38

Part 1Input Sequence

           10
 1 EPPRVKRYIEIQCLD

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	2	4, 7
Asp-N endopeptidase	1	14
Asp-N endopeptidase + N-terminal Glu	2	9, 14
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	8
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	2	8, 14
Clostripain (Clostridiopeptidase B)	2	4, 7
Formic acid	1	15
Glutamyl endopeptidase	2	1, 10
LysC	1	6
LysN	1	5
Neutrophil elastase	1	5
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	12
Pepsin (pH1.3)	1	13
Pepsin (pH>2)	1	13
Proteinase K	7	1, 5, 8, 9, 10, 11, 14
Staphylococcal peptidase I	1	10
Thermolysin	3	4, 8, 13
Trypsin	3	4, 6, 7

The selected enzymes do not cut: BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, CNBr, Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                        HCOOH|
               AspN_AspN_AspGluN_Ch_lo_ProtK||
                           Pn1.3_Pn2p_Therm|||
                                      NTCB||||
                                    ProtK|||||
                        GluC_ProtK_Staph||||||
                     AspN_AspGluN_ProtK|||||||
               Ch_hi_Ch_lo_ProtK_Therm||||||||
                     ArgC_Clost_Tryps|||||||||
                          LysC_Tryps||||||||||
                   LysN_Elast_ProtK|||||||||||
            ArgC_Clost_Therm_Tryps||||||||||||
                     GluC_ProtK|  ||||||||||||
                               EPPRVKRYIEIQCLD
                           1   ---------+-----   15

seq_9

Length 15 aa · Enzymes 38

Part 1Input Sequence

           10
 1 RMMPRQNVGHAAQNH

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	2	1, 5
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	2	2, 10
Clostripain (Clostridiopeptidase B)	2	1, 5
CNBr	2	2, 3
Neutrophil elastase	3	8, 11, 12
Proteinase K	3	8, 11, 12
Thermolysin	4	1, 7, 10, 11
Trypsin	2	1, 5

The selected enzymes do not cut: Asp-N endopeptidase, Asp-N endopeptidase + N-terminal Glu, BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Chymotrypsin-high specificity (C-term to [FYW], not before P), Enterokinase, Factor Xa, Formic acid, Glutamyl endopeptidase, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, LysC, LysN, NTCB (2-nitro-5-thiocyanobenzoic acid), Pepsin (pH1.3), Pepsin (pH>2), Proline-endopeptidase[*], Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                        Elast_ProtK|
                 Elast_ProtK_Therm||
                      Ch_lo_Therm|||
                    Elast_ProtK| |||
                         Therm|| |||
            ArgC_Clost_Tryps| || |||
                      CNBr| | || |||
               Ch_lo_CNBr|| | || |||
  ArgC_Clost_Therm_Tryps||| | || |||
                        RMMPRQNVGHAAQNH
                    1   ---------+-----   15

seq_10

Length 15 aa · Enzymes 38

Part 1Input Sequence

           10
 1 DYRNGVPKCADANCK

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	3
Asp-N endopeptidase	1	10
Asp-N endopeptidase + N-terminal Glu	1	10
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	2
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	1	2
Clostripain (Clostridiopeptidase B)	1	3
Formic acid	2	1, 11
Hydroxylamine (NH2OH)	1	4
LysC	2	8, 15
LysN	2	7, 14
Neutrophil elastase	3	6, 10, 12
NTCB (2-nitro-5-thiocyanobenzoic acid)	2	8, 13
Proteinase K	4	2, 6, 10, 12
Thermolysin	1	9
Trypsin	2	3, 8

The selected enzymes do not cut: BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, CNBr, Enterokinase, Factor Xa, Glutamyl endopeptidase, GranzymeB, Iodosobenzoic acid, Pepsin (pH1.3), Pepsin (pH>2), Proline-endopeptidase[*], Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                         LysC|
                                        LysN||
                                       NTCB|||
                               Elast_ProtK||||
                                    HCOOH|||||
           AspN_AspN_AspGluN_Elast_ProtK||||||
                                  Therm|||||||
                       LysC_NTCB_Tryps||||||||
                                 LysN|||||||||
                         Elast_ProtK||||||||||
                             Hydro| ||||||||||
                 ArgC_Clost_Tryps|| ||||||||||
               Ch_hi_Ch_lo_ProtK||| ||||||||||
                          HCOOH|||| ||||||||||
                               DYRNGVPKCADANCK
                           1   ---------+-----   15

seq_11

Length 10 aa · Enzymes 38

Part 1Input Sequence

           10
 1 HVRYEHWAGI

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	3
Asp-N endopeptidase + N-terminal Glu	1	4
BNPS-Skatole	1	7
Chymotrypsin-high specificity (C-term to [FYW], not before P)	2	4, 7
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	3	1, 4, 7
Clostripain (Clostridiopeptidase B)	1	3
Glutamyl endopeptidase	1	5
Iodosobenzoic acid	1	7
Neutrophil elastase	2	2, 8
Pepsin (pH>2)	3	4, 6, 7
Proteinase K	6	2, 4, 5, 7, 8, 10
Staphylococcal peptidase I	1	5
Thermolysin	2	1, 7
Trypsin	1	3

The selected enzymes do not cut: Asp-N endopeptidase, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, CNBr, Enterokinase, Factor Xa, Formic acid, GranzymeB, Hydroxylamine (NH2OH), LysC, LysN, NTCB (2-nitro-5-thiocyanobenzoic acid), Pepsin (pH1.3), Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                            ProtK|
                                    Elast_ProtK| |
        BNPS_Ch_hi_Ch_lo_Iodo_Pn2p_ProtK_Therm|| |
                                         Pn2p||| |
                            GluC_ProtK_Staph|||| |
        AspN_AspGluN_Ch_hi_Ch_lo_Pn2p_ProtK||||| |
                          ArgC_Clost_Tryps|||||| |
                              Elast_ProtK||||||| |
                             Ch_lo_Therm|||||||| |
                                        HVRYEHWAGI
                                    1   ---------+   10

seq_12

Length 13 aa · Enzymes 38

Part 1Input Sequence

           10
 1 FADYDVWHGWQKE

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase	2	2, 4
Asp-N endopeptidase + N-terminal Glu	3	2, 4, 12
BNPS-Skatole	2	7, 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)	4	1, 4, 7, 10
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	5	1, 4, 7, 8, 10
Formic acid	2	3, 5
Glutamyl endopeptidase	1	13
Iodosobenzoic acid	2	7, 10
LysC	1	12
LysN	1	11
Neutrophil elastase	2	2, 6
Pepsin (pH>2)	5	3, 4, 6, 7, 9
Proteinase K	7	1, 2, 4, 6, 7, 10, 13
Staphylococcal peptidase I	1	13
Thermolysin	1	1
Trypsin	1	12

The selected enzymes do not cut: Arg-C proteinase, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), CNBr, Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), NTCB (2-nitro-5-thiocyanobenzoic acid), Pepsin (pH1.3), Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                      GluC_ProtK_Staph|
                              AspN_AspGluN_LysC_Tryps||
                                                LysN|||
                        BNPS_Ch_hi_Ch_lo_Iodo_ProtK||||
                                              Pn2p|||||
                                            Ch_lo||||||
                BNPS_Ch_hi_Ch_lo_Iodo_Pn2p_ProtK|||||||
                               Elast_Pn2p_ProtK||||||||
                                         HCOOH|||||||||
     AspN_AspN_AspGluN_Ch_hi_Ch_lo_Pn2p_ProtK||||||||||
                                  HCOOH_Pn2p|||||||||||
              AspN_AspN_AspGluN_Elast_ProtK||||||||||||
                   Ch_hi_Ch_lo_ProtK_Therm|||||||||||||
                                          FADYDVWHGWQKE
                                      1   ---------+---   13

seq_13

Length 14 aa · Enzymes 38

Part 1Input Sequence

           10
 1 TDGLYWAEMACQLP

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase	1	1
Asp-N endopeptidase + N-terminal Glu	2	1, 7
BNPS-Skatole	1	6
Chymotrypsin-high specificity (C-term to [FYW], not before P)	2	5, 6
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	4	4, 5, 6, 9
CNBr	1	9
Formic acid	1	2
Glutamyl endopeptidase	1	8
Iodosobenzoic acid	1	6
Neutrophil elastase	2	7, 10
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	10
Pepsin (pH1.3)	2	3, 4
Pepsin (pH>2)	4	3, 4, 5, 6
Proteinase K	8	1, 4, 5, 6, 7, 8, 10, 13
Staphylococcal peptidase I	1	8
Thermolysin	3	3, 6, 9

The selected enzymes do not cut: Arg-C proteinase, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), LysC, LysN, Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease, Trypsin

Part 4Cleavage Mapping

                                               ProtK|
                                 Elast_NTCB_ProtK|  |
                                Ch_lo_CNBr_Therm||  |
                               GluC_ProtK_Staph|||  |
                      AspN_AspGluN_Elast_ProtK||||  |
       BNPS_Ch_hi_Ch_lo_Iodo_Pn2p_ProtK_Therm|||||  |
                      Ch_hi_Ch_lo_Pn2p_ProtK||||||  |
                     Ch_lo_Pn1.3_Pn2p_ProtK|||||||  |
                          Pn1.3_Pn2p_Therm||||||||  |
                                    HCOOH|||||||||  |
                 AspN_AspN_AspGluN_ProtK||||||||||  |
                                        TDGLYWAEMACQLP
                                    1   ---------+----   14

seq_14

Length 19 aa · Enzymes 38

Part 1Input Sequence

           10
 1 KNQYRSDKFWAKPRNEGCE

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	2	5, 14
Asp-N endopeptidase	1	6
Asp-N endopeptidase + N-terminal Glu	3	6, 15, 18
BNPS-Skatole	1	10
Chymotrypsin-high specificity (C-term to [FYW], not before P)	3	4, 9, 10
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	3	4, 9, 10
Clostripain (Clostridiopeptidase B)	2	5, 14
Formic acid	1	7
Glutamyl endopeptidase	2	16, 19
Iodosobenzoic acid	1	10
LysC	3	1, 8, 12
LysN	2	7, 11
Neutrophil elastase	1	11
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	17
Pepsin (pH1.3)	2	8, 9
Pepsin (pH>2)	3	4, 8, 9
Proline-endopeptidase[*]	1	13
Proteinase K	6	4, 9, 10, 11, 16, 19
Staphylococcal peptidase I	2	16, 19
Thermolysin	2	8, 10
Trypsin	4	1, 5, 8, 14

The selected enzymes do not cut: Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, CNBr, Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                     GluC_ProtK_Staph|
                                        AspN_AspGluN||
                                               NTCB|||
                                  GluC_ProtK_Staph||||
                                     AspN_AspGluN|||||
                                ArgC_Clost_Tryps||||||
                                           Prol|||||||
                                          LysC||||||||
                             LysN_Elast_ProtK|||||||||
           BNPS_Ch_hi_Ch_lo_Iodo_ProtK_Therm||||||||||
               Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK|||||||||||
               LysC_Pn1.3_Pn2p_Therm_Tryps||||||||||||
                               HCOOH_LysN|||||||||||||
                       AspN_AspN_AspGluN||||||||||||||
                       ArgC_Clost_Tryps|||||||||||||||
                Ch_hi_Ch_lo_Pn2p_ProtK||||||||||||||||
                         LysC_Tryps|  ||||||||||||||||
                                   KNQYRSDKFWAKPRNEGCE
                               1   ---------+---------   19

seq_15

Length 11 aa · Enzymes 38

Part 1Input Sequence

           10
 1 VNLRVWFNSMA

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	4
BNPS-Skatole	1	6
Chymotrypsin-high specificity (C-term to [FYW], not before P)	2	6, 7
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	4	3, 6, 7, 10
Clostripain (Clostridiopeptidase B)	1	4
CNBr	1	10
Iodosobenzoic acid	1	6
Neutrophil elastase	3	1, 5, 11
Pepsin (pH1.3)	2	3, 7
Pepsin (pH>2)	3	3, 5, 7
Proteinase K	6	1, 3, 5, 6, 7, 11
Thermolysin	4	2, 4, 6, 9
Trypsin	1	4

The selected enzymes do not cut: Asp-N endopeptidase, Asp-N endopeptidase + N-terminal Glu, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Enterokinase, Factor Xa, Formic acid, Glutamyl endopeptidase, GranzymeB, Hydroxylamine (NH2OH), LysC, LysN, NTCB (2-nitro-5-thiocyanobenzoic acid), Proline-endopeptidase[*], Staphylococcal peptidase I, Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                  Elast_ProtK|
                                  Ch_lo_CNBr||
                                      Therm|||
             Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK| |||
       BNPS_Ch_hi_Ch_lo_Iodo_ProtK_Therm|| |||
                       Elast_Pn2p_ProtK||| |||
                ArgC_Clost_Therm_Tryps|||| |||
               Ch_lo_Pn1.3_Pn2p_ProtK||||| |||
                               Therm|||||| |||
                        Elast_ProtK||||||| |||
                                   VNLRVWFNSMA
                               1   ---------+-   11

seq_16

Length 15 aa · Enzymes 38

Part 1Input Sequence

           10
 1 NCGLSEHEHDANIFV

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase	1	9
Asp-N endopeptidase + N-terminal Glu	3	5, 7, 9
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	14
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	3	4, 7, 14
Formic acid	1	10
Glutamyl endopeptidase	2	6, 8
Neutrophil elastase	2	11, 15
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	1
Pepsin (pH1.3)	3	3, 4, 13
Pepsin (pH>2)	3	3, 4, 13
Proteinase K	7	4, 6, 8, 11, 13, 14, 15
Staphylococcal peptidase I	2	6, 8
Thermolysin	3	3, 12, 13

The selected enzymes do not cut: Arg-C proteinase, BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), CNBr, Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, LysC, LysN, Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease, Trypsin

Part 4Cleavage Mapping

                           Elast_ProtK|
                    Ch_hi_Ch_lo_ProtK||
              Pn1.3_Pn2p_ProtK_Therm|||
                              Therm||||
                       Elast_ProtK|||||
                            HCOOH||||||
               AspN_AspN_AspGluN|||||||
               GluC_ProtK_Staph||||||||
            AspN_AspGluN_Ch_lo|||||||||
             GluC_ProtK_Staph||||||||||
                AspN_AspGluN|||||||||||
     Ch_lo_Pn1.3_Pn2p_ProtK||||||||||||
          Pn1.3_Pn2p_Therm|||||||||||||
                    NTCB| |||||||||||||
                        NCGLSEHEHDANIFV
                    1   ---------+-----   15

seq_17

Length 14 aa · Enzymes 38

Part 1Input Sequence

           10
 1 FSCRPCQESAVPEP

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	4
Asp-N endopeptidase + N-terminal Glu	2	7, 12
Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	1
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	1	1
Clostripain (Clostridiopeptidase B)	1	4
Glutamyl endopeptidase	2	8, 13
Neutrophil elastase	2	10, 11
NTCB (2-nitro-5-thiocyanobenzoic acid)	2	2, 5
Proline-endopeptidase[*]	1	5
Proteinase K	5	1, 8, 10, 11, 13
Staphylococcal peptidase I	2	8, 13
Thermolysin	1	9

The selected enzymes do not cut: Asp-N endopeptidase, BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, CNBr, Enterokinase, Factor Xa, Formic acid, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, LysC, LysN, Pepsin (pH1.3), Pepsin (pH>2), Thrombin, Tobacco etch virus protease, Trypsin

Part 4Cleavage Mapping

               GluC_ProtK_Staph|
                  AspN_AspGluN||
                  Elast_ProtK|||
                 Elast_ProtK||||
                      Therm|||||
          GluC_ProtK_Staph||||||
             AspN_AspGluN|||||||
              NTCB_Prol| |||||||
            ArgC_Clost|| |||||||
                NTCB| || |||||||
  Ch_hi_Ch_lo_ProtK|| || |||||||
                   FSCRPCQESAVPEP
               1   ---------+----   14

seq_18

Length 16 aa · Enzymes 38

Part 1Input Sequence

           10
 1 WHFHTFLNREMEFCVF

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Arg-C proteinase	1	9
Asp-N endopeptidase + N-terminal Glu	2	9, 11
BNPS-Skatole	1	1
Chymotrypsin-high specificity (C-term to [FYW], not before P)	4	1, 3, 6, 13
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	8	1, 2, 3, 4, 6, 7, 11, 13
Clostripain (Clostridiopeptidase B)	1	9
CNBr	1	11
Glutamyl endopeptidase	2	10, 12
Iodosobenzoic acid	1	1
Neutrophil elastase	1	15
NTCB (2-nitro-5-thiocyanobenzoic acid)	1	13
Pepsin (pH1.3)	5	3, 5, 7, 12, 13
Pepsin (pH>2)	5	3, 5, 7, 12, 13
Proteinase K	10	1, 3, 5, 6, 7, 10, 12, 13, 15, 16
Staphylococcal peptidase I	2	10, 12
Thermolysin	4	2, 5, 6, 14
Trypsin	1	9

The selected enzymes do not cut: Asp-N endopeptidase, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Enterokinase, Factor Xa, Formic acid, GranzymeB, Hydroxylamine (NH2OH), LysC, LysN, Proline-endopeptidase[*], Thrombin, Tobacco etch virus protease

Part 4Cleavage Mapping

                                             ProtK|
                                      Elast_ProtK||
                                           Therm|||
              Ch_hi_Ch_lo_NTCB_Pn1.3_Pn2p_ProtK||||
                   GluC_Pn1.3_Pn2p_ProtK_Staph|||||
                      AspN_AspGluN_Ch_lo_CNBr||||||
                            GluC_ProtK_Staph|||||||
              ArgC_AspN_AspGluN_Clost_Tryps||||||||
                   Ch_lo_Pn1.3_Pn2p_ProtK| ||||||||
                 Ch_hi_Ch_lo_ProtK_Therm|| ||||||||
                 Pn1.3_Pn2p_ProtK_Therm||| ||||||||
                                 Ch_lo|||| ||||||||
         Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK||||| ||||||||
                         Ch_lo_Therm|||||| ||||||||
        BNPS_Ch_hi_Ch_lo_Iodo_ProtK||||||| ||||||||
                                   WHFHTFLNREMEFCVF
                               1   ---------+------   16

seq_19

Length 16 aa · Enzymes 38

Part 1Input Sequence

           10
 1 VYTNFDFYVQGYKIME

Part 2Selected Enzymes

Selected cleavage enzymes and chemicals.

Arg-C proteinase
Asp-N endopeptidase
Asp-N endopeptidase + N-terminal Glu
BNPS-Skatole
Caspase 1
Caspase 2
Caspase 3
Caspase 4
Caspase 5
Caspase 6
Caspase 7
Caspase 8
Caspase 9
Caspase 10
Chymotrypsin-high specificity (C-term to [FYW], not before P)
Chymotrypsin-low specificity (C-term to [FYWML], not before P)
Clostripain (Clostridiopeptidase B)
CNBr
Enterokinase
Factor Xa
Formic acid
Glutamyl endopeptidase
GranzymeB
Hydroxylamine (NH2OH)
Iodosobenzoic acid
LysC
LysN
Neutrophil elastase
NTCB (2-nitro-5-thiocyanobenzoic acid)
Pepsin (pH1.3)
Pepsin (pH>2)
Proline-endopeptidase[*]
Proteinase K
Staphylococcal peptidase I
Thermolysin
Thrombin
Tobacco etch virus protease
Trypsin

[*] NOTE: Proline-endopeptidase was reported to cleave only substrates whose sequences do not exceed 30 amino acids. An unusual beta-propeller domain regulates proteolysis: see Fulop et al., 1998.

Part 3Cleavage Site Table

Cleavage site table
Name of enzyme	No. of cleavages	Positions of cleavage sites
Asp-N endopeptidase	1	5
Asp-N endopeptidase + N-terminal Glu	2	5, 15
Chymotrypsin-high specificity (C-term to [FYW], not before P)	5	2, 5, 7, 8, 12
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	6	2, 5, 7, 8, 12, 15
CNBr	1	15
Formic acid	1	6
Glutamyl endopeptidase	1	16
LysC	1	13
LysN	1	12
Neutrophil elastase	2	1, 9
Pepsin (pH1.3)	4	4, 5, 6, 7
Pepsin (pH>2)	7	4, 5, 6, 7, 8, 11, 12
Proteinase K	10	1, 2, 3, 5, 7, 8, 9, 12, 14, 16
Staphylococcal peptidase I	1	16
Thermolysin	4	4, 8, 13, 14
Tobacco etch virus protease	1	10
Trypsin	1	13

The selected enzymes do not cut: Arg-C proteinase, BNPS-Skatole, Caspase 1, Caspase 2, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Clostripain (Clostridiopeptidase B), Enterokinase, Factor Xa, GranzymeB, Hydroxylamine (NH2OH), Iodosobenzoic acid, NTCB (2-nitro-5-thiocyanobenzoic acid), Proline-endopeptidase[*], Thrombin

Part 4Cleavage Mapping

                                               GluC_ProtK_Staph|
                                       AspN_AspGluN_Ch_lo_CNBr||
                                                  ProtK_Therm|||
                                            LysC_Therm_Tryps||||
                                Ch_hi_Ch_lo_LysN_Pn2p_ProtK|||||
                                                      Pn2p||||||
                                                      TEV|||||||
                                             Elast_ProtK||||||||
                           Ch_hi_Ch_lo_Pn2p_ProtK_Therm|||||||||
                          Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK||||||||||
                                     HCOOH_Pn1.3_Pn2p|||||||||||
      AspN_AspN_AspGluN_Ch_hi_Ch_lo_Pn1.3_Pn2p_ProtK||||||||||||
                                   Pn1.3_Pn2p_Therm|||||||||||||
                                             ProtK||||||||||||||
                                Ch_hi_Ch_lo_ProtK|||||||||||||||
                                     Elast_ProtK||||||||||||||||
                                                VYTNFDFYVQGYKIME
                                            1   ---------+------   16